307 IN VITRO PRODUCTION OF BOVINE EMBRYOS USING FLOW-CYTOMETRICALLY SORTED SPERMATOZOA
نویسندگان
چکیده
منابع مشابه
In vitro production of bovine embryos using flow-cytometrically sexed sperm
The investigation aimed to compare the effect of fresh and frozen-thawed X and Y fractions of flowcytometrically sorted bovine spermatozoa on in vitro fertilization of bovine in vitro matured oocytes and subsequent blastocyst development. Sperm cells sorted in MoFloSX cytometer were used either for IVF or frozen and stored in liquid nitrogen. Immature oocytes recovered from ovaries of slaughter...
متن کاملApplication and commercialization of flow cytometrically sex-sorted semen.
The current technology to sort X and Y chromosome bearing sperm population requires individual identification and selection of spermatozoa in a modified high-speed flow cytometer. For farm animal species, the technology is capable of producing sexed sperm at greater than 90% purity. However, only in the bovine, the technology has reached a developmental level that allows its commercial applicat...
متن کاملIn vitro and in vivo quality of bovine embryos in vitro produced with sex-sorted sperm.
In this work we analyzed the effects of three culture systems on developmental ability of bovine embryos in vitro produced with sexed sperm, the survival to vitrification (cryologic vitrification method) of such blastocysts, and their pregnancy rates after embryo transfer to recipients, both as fresh and after vitrification/warming. Finally, we measured the accuracy of the sorting protocol by a...
متن کاملFluorescence in situ hybridization in diluted and flow cytometrically sorted boar spermatozoa using specific DNA direct probes labelled by nick translation.
Successful evaluation of X- and Y-chromosome-bearing sperm separation technology using flow cytometry-cell sorter is of great importance. Fluorescence in situ hybridization (FISH), which allows for the detection of specific nucleic acid sequences on morphologically preserved spermatozoa, is an ideal method for quantitatively and qualitatively assessing the purity of sorted sperm samples. In thi...
متن کاملFourier Transform Infrared spectroscopy discloses different types of cell death in flow cytometrically sorted cells.
Fourier Transform Infrared (FTIR) spectroscopy is a label free methodology showing promise in characterizing different types of cell death. Cervical adenocarcinoma (HeLa) and African monkey kidney (Vero) cells were treated with a necrosis inducer (methanol), novel apoptotic inducers (diphenylphosphino gold (I) complexes) and positive control, auranofin. Following treatment, cells stained with a...
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ژورنال
عنوان ژورنال: Reproduction, Fertility and Development
سال: 2005
ISSN: 1031-3613
DOI: 10.1071/rdv17n2ab307